Real-time whole slide pathology image cell counting

A non-transitory, computer-readable medium storing instructions which, when executed by at least one processor and memory, cause the at least one processor to perform operations comprising one or more steps to:

• obtain, in at least one computer-readable memory, a whole image comprising images of objects;
• segment the whole image into image areas based on a global threshold;
• select a plurality of patches associated with the image areas;
• generate an intensity image based on an optical density of each patch of the plurality of patches;
• generate at least one object mask with at least one object seed by segmenting the intensity image based on an iteratively reducing threshold;
• generate a distance map based on at least one transformation of the at least one object mask;
• distinguish object segments within the plurality of patches based on at least one transformation of the distance map and at least one object seed;
• and
• configure a device to display an object count for at least one patch in the plurality of patches based on counting the cell segments.

A computerized method comprising:

• obtaining, in at least one computer-readable memory, a whole image comprising images of objects;
• segmenting the whole image into image areas based on a global threshold;
• selecting a plurality of patches associated with the image areas;
• generating an intensity image based on an optical density of each patch of the plurality of patches;
• generating at least one object mask with at least one object seed by segmenting the intensity image based on an iteratively reducing threshold;
• generating a distance map based on at least one transformation of the at least one object mask;
• distinguishing object segments within the plurality of patches based on at least one transformation of the distance map and at least one object seed;
• and
• configuring a device to display an object count for at least one patch in the plurality of patches based on counting the object segments.