Method and kit for monitoring anticoagulant therapy

A test kit for measuring activity of an anticoagulant drug in a patient, the test kit comprising:

• a coagulation reagent, and
• a factor-deficient plasma deficient in vitamin K dependent factors II, IX and X;
• and
• wherein the factor-deficient plasma includes at least 0.5 μg/mL of factor VII.

A method for monitoring anticoagulant therapy, the method comprising:

• combining adsorbed plasma with a solution containing factor VII to correct a final concentration of factor VII in a resulting sample mixture, the adsorbed plasma being deficient in vitamin K dependent factors II, VII, X, and IX, the final concentration of factor VII being corrected to over 50% or greater than 0.5 u/mL, the resulting sample mixture being deficient in only factors II, X and IX;
• adding a coagulation reagent to the resulting sample mixture to initiate a clotting reaction, wherein the coagulation reagent directly activates factor X and does not activate factor IX, bypassing activation of factor IX;
• measuring simultaneous influence of factors II and X on the clotting reaction;
• and
• determining blood clotting ability of the resulting sample mixture by measuring blood clotting time or thrombin generation.