Low temperature oligosaccharide analytical system and method
AssigneeDionex Corporation
InventorsTian Tian, Yan Liu
Life of this patent
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Abstract
A novel analytical system and method to analyze complex carbohydrates such as human milk oligosaccharides by low-temperature High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection and High-resolution Mass Spectrometry (HPAE-PAD-MS). The analytical system controls the temperature of the column, electrochemical detector, and ion removal device at or below 15° C. The HPAE-PAD workflow with high-resolution mass spectrometry provides useful molecular structure information. It facilitates the detection of milk oligosaccharides, particularly unknown structures, without the use of analytical standards.
Claims9
A method of analyzing an oligosaccharide sample, the method comprising:
- injecting the oligosaccharide sample into a chromatography column of the chromatography system;
- flowing a mobile phase into the chromatography column to separate the oligosaccharide sample into one or more analytes that elute off the chromatography column at different times;
- flowing the mobile phase from the chromatography column into an electrochemical detector to detect one or more analytes;
- flowing the mobile phase from the electrochemical detector into an ion removal device;
- removing at least ions of one charge from the mobile phase in the ion removal device;
- and
- flowing the mobile phase to a mass spectrometer;
- wherein the chromatography column, electrochemical detector, and the ion removal device are all at a temperature of 15° C. or less;
- wherein less than 5% of the oligosaccharide sample is epimerized.
Dependencies are inferred from the claim text.