Back to results
US 12,404,503 B2Granted
Methods and compositions for identifying epitopes
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsStephen Elledge, Tomasz Kula, Mohammad Haj Dezfulian
Life of this patent
Estimating the term…
Abstract
Described herein are methods for identifying immune cell-specific antigens and compositions for use in the methods.
Claims22
A system for detection of recognized antigen presentation by an antigen presenting cell to a cytotoxic lymphocyte or NK cell comprising:
- a) an antigen presenting cell (APC) comprising:
- i. an exogenous nucleic acid encoding a candidate antigen, wherein the candidate antigen is expressed and presented with MHC class I and/or MHC class II molecules to a cytotoxic lymphocyte and/or NK cell;
- ii. a molecular reporter of Granzyme B (GzB) activity;
- and
- iii. (1) an exogenous nucleic acid encoding inhibitor of caspase-activated deoxyribonuclease (ICAD) in expressible form, (2) an exogenous inhibitory nucleic acid targeting CAD, (3) a CAD knockout, or (4) a caspase knockout;
- and
- b) a cytotoxic lymphocyte and/or NK cell.
4 more elements
A system for detection of recognized antigen presentation by an antigen presenting cell to a cytotoxic lymphocyte or NK cell comprising:
- a) an antigen presenting cell (APC) comprising:
- i. an exogenous nucleic acid encoding a candidate antigen, wherein the candidate antigen is expressed and presented with MHC class I and/or MHC class II molecules to a cytotoxic lymphocyte and/or NK cell;
- ii. a molecular reporter of Granzyme B (GzB) activity;
- and
- iii. an inhibitor of CAD-mediated degradation;
- and
- b) a cytotoxic lymphocyte and/or NK cell, wherein the cytotoxic lymphocyte and/or NK cell is cytotoxic T cell and/or NK cell that has been modified to express a T cell receptor from a non-cytotoxic CD4 T cell.
4 more elements
Dependencies are inferred from the claim text.