Method of cell lysis

A method of cell lysis for forming at least one pore in a cell wall and/or cell membrane of a cell in an aqueous medium for release of at least one intracellular target protein and/or lipid from the cell into the aqueous medium, the method comprising:

• (a) subjecting the cell with the at least one intracellular target protein and/or lipid in the aqueous medium to an electrohydraulic comminution;
• and
• (b) separating the at least one intracellular target protein and/or lipid from the cell,
• wherein the electrohydraulic comminution is brought about by means of a pulse current source, the cell in the aqueous medium is subjected to electrical impulse discharges between at least two electrodes of an underwater radio path of a container at an operating voltage in the range from 40-130 kV, an impulse energy of 50 to 1500 J per electrode, a pulse frequency of 1 Hz for 40-1000 seconds, whereby at least one pore is formed in the cell wall and/or cell membrane.