Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

A genetically modified host cell comprising:

• a heterologous nucleic acid encoding a fusion protein comprising a protein of interest fused in frame to a maltose dependent degron,
• wherein the fusion protein is most stable when the maltose dependent degron is in contact with a maltose based inducer compared to when the maltose dependent degron is not in contact with the maltose based inducer,
• wherein the maltose degron in the fusion protein comprises:
• i. the amino acid sequence of SEQ ID NO:
• 2 or SEQ ID NO:
• 28;
• ii. amino acid residues 1-365 of the amino acid sequence of SEQ ID NO:
• 2 or SEQ ID NO:
• 28;
• or
• iii. a homolog comprising at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
• 2 or SEQ ID NO:
• 28;
• and
• wherein the maltose dependent degron further comprises one or more variant amino acid residue substitutions selected from the group consisting of K7R, I10T, W11G, L21S, V24A, F28Y, D42V, K43E, A64T, F68S, D83G, D88N, P92T, W95R, V98I, N101I, A110T, I117V, P134S, A135T, L136M, M149I, Y168C, Y168N, Y177H, N186S, A187P, L193S, D198V, D210E, A216V, A217D, G229C, I236N, D237N, N242D, L263M, L291V, A304S, T321N, M322L, A339T, A351T, T357S, T367S, S370P, and N374S, wherein said one or more variant amino acid residue substitutions correspond to the amino acid positions of the amino acid sequence of SEQ ID NO:
• 2 or SEQ ID NO:
• 28.