Methods and compositions for use of recombinant bacterial effector proteins as anti-inflammatory agents
AssigneesThe Wistar Institute of Anatomy and Biology, Innate Biologics LLC
InventorsFrank J. Rauscher, Peter Mondics
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Abstract
Provided herein are methods and compositions comprising a set of paired peptides comprising a first bacterial effector polypeptide or fragment thereof linked to a second bacterial effector polypeptide or fragment thereof. The paired peptides can be linked to a protein transduction domain. The compositions can be formulated as pharmaceuticals. The compositions are useful for the treatment of inflammatory disorders.
Claims12
A composition comprising a set of paired peptides, wherein the set of paired peptides is linked to a protein transduction domain, and wherein the set of paired peptides comprises a first bacterial effector polypeptide linked to a second bacterial effector polypeptide, wherein the protein transduction domain is a YopM protein transduction domain, an SspH1 protein transduction domain, or an 1paH protein transduction domain wherein the protein transduction domain and the set of paired peptides comprise a fusion protein and the amino acid sequence of the fusion protein is at least 85% identical to the sequence set forth in SEQ ID NO. 10, 13, 16, 19, 22, or 24.
A fusion protein comprising a set of paired peptides, wherein the set of paired peptides comprises a first bacterial effector polypeptide linked to a second bacterial effector polypeptide wherein the amino acid sequence of the fusion protein is at least 85% identical to the sequence set forth in SEQ ID NO. 10, 13, 16, 19, 22, or 24.
A composition comprising a protein transduction domain polypeptide linked to a first bacterial effector polypeptide and at least one additional bacterial effector polypeptides wherein the first bacterial effector polypeptide is a polypeptide having 90% sequence identity to an amino acid sequence set forth in the group consisting of SEQ ID NOs 3, 89, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, and 79 and the at least one additional effector polypeptide is a polypeptide having 90% sequence identity to an amino acid sequence set forth in the group consisting of SEQ ID NOs. 3, 89, 42, 44,46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, and 79.
Dependencies are inferred from the claim text.