Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins

A solid-phase chelator material comprising a solid phase, polyamine groups bound to the solid phase and chelating groups bound to the polyamine groups, wherein at least a part of the polyamine groups are connected with at least two chelating groups per polyamine group and wherein each chelating group comprises one or several aminopolycarboxylic acid groups (APA groups), with the proviso that the number of APA groups per polyamine group connected with at least two chelating groups is at least three,

• wherein the APA groups are derived from the APAs which are selected from the group consisting of ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, triethylenetetraminehexaacetic acid, ethylenediamine-N,N′-disuccinic acid, ethyleneglycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid, and 1,4,7-triazacyclononane-1,4,7-trisacetic acid, and
• wherein the polyamine groups are derived from the polyamines which are selected from the group consisting of diethylene triamine, triethylene tetramine, tetraethylene pentamine, penta ethylene hexamine, linear and branched polyethylene imine, poly-lysine, polypropylene imine, macrocylic polyamines cyclam and cyclen, the branched polyamines 1,1,1-tris(aminomethyl)ethane, tris-aminoethyl amine, tris aminopropyl amine, tetrakis-ethylamino amine.