A method for producing a plant-based extract composition having the effect of enhancing nutrition and immunity, comprising the following steps (i) to (viii):
- (i) preparing materials including:
- (a) a Ngoc Linh ginseng extract component having a predetermined first percentage (%) by weight, prepared by mixing a first extract and a second extract at a ratio of 2:(0.05-1) v/v;
- wherein the first extract is prepared by performing steps (a1) to (c1), comprising:
- (a1) preparing material:
- collecting Ngoc Linh ginseng roots aged 3-5 years, cleaning, cutting into pieces, drying to a moisture content below 15%, grinding, and sieving through a 0.5-1 mm mesh to obtain a first powder;
- (b1) extracting the first powder with saturated n-butanol (n-BuOH) solution at a ratio of 1:20 w/v for 1 hour, followed by ultrasonic-assisted extraction at a frequency of 35 kHz for 2 hours, wherein step (b1) is repeated three times to obtain a first temporary extract;
- and
- (c1) removing the solvent from the first temporary extract and centrifuging at a speed of 1000-5000 rpm for 10 minutes to obtain the first extract;
- wherein the second extract is prepared by performing steps (a2) to (d2), comprising:
- (a2) preparing material:
- collecting the stems and leaves of the Ngoc Linh ginseng aged 3-5 years, cleaning, cutting into pieces, drying to a moisture content below 15%, grinding, and sieving through a 0.5-1 mm mesh to obtain a second powder;
- (b2) extracting the second powder with 70% ethanol solution at a ratio of 1:8 w/v for 2 days, then filtering to obtain a second temporary extract and a first residue;
- (c2) extracting the first residue with 70% ethanol solution at a ratio of 1:2 w/v using ultrasonic-assisted extraction at a frequency of 35 kHz for 3 hours, then filtering to obtain a third temporary extract;
- and
- (d2) mixing the second temporary extract with the third temporary extract, removing the solvent, and filtering to obtain the second extract;
- (b) a jiaogulan powder component having a predetermined second percentage (%) by weight, prepared by cleaning leaves and stems of the Gynostemma pentaphyllum, cold-drying at 30° C.-31° C. until the moisture reaches 5%-7%, then grinding and sieving through a 0.5-1 mm mesh to obtain the jiaogulan powder component;
- (c) a maingay's tree powder component having a predetermined third percentage (%) by weight, prepared by cleaning the Cratoxylum maingayi leaves, drying at 50° C.-60° C. until the moisture reaches 5%-7%, then grinding and sieving through a 0.5-1 mm mesh to obtain maingay's tree powder component;
- (d) a nodding clubmoss powder component having a predetermined fourth percentage (%) by weight, prepared by cleaning the entire plant of the Lycopodiella cernua, drying at 50° C.-60° C. until moisture reaches 5%-7%, then grinding and sieving through a 0.5-1 mm mesh to obtain the nodding clummoss powder component;
- (e) a red ginseng powder component having a predetermined fifth percentage (%) by weight, prepared by cleaning red ginseng roots, drying in three stages, then grinding and sieving through a 0.5-1 mm mesh to obtain the red ginseng powder component;
- wherein drying in three stages includes:
- a first stage:
- drying at 40° C. for 24 hours:
- a second stage:
- drying at 50° C. for 48 hours;
- and
- a third stage:
- drying at 60° C. until the moisture reaches 5%-7%;
- (f) a codonopsis root powder component having a predetermined sixth percentage (%) by weight, prepared by cleaning the Codonopsis root, cutting into 0.5-1 cm pieces, drying at 60° C. until the moisture reaches 5%-7%, then grinding and sieving through a 0.5-1 mm mesh to obtain the codonopsis root powder component;
- (g) an angelica root powder component having a predetermined seventh percentage (%) by weight, prepared by cleaning the Angelica root, drying at 30° C.-35° C. until the moisture reaches 5%-7%, then grinding and sieving through a 0.5-1 mm mesh to obtain the angelica root powder component;
- (h) a rice bran oil component having a predetermined eighth percentage (%) by weight, extracted from germ and inner bran layer of rice using water-assisted ultrasonic extraction at 50° C. for 30 minutes with a stirring speed of 1000 rpm and a rice bran-to-water ratio of 1.5:10;
- ultrasonic treatment is conducted for 70 minutes at 25° C. to obtain the rice bran oil component;
- (i) a legume powder component having a predetermined ninth percentage (%) by weight, including 1 part red bean, 1 part black bean, 1 part green bean, 1 part soybean, and 1 part white bean;
- (j) a coenzyme Q10 component having a predetermined tenth percentage (%) by weight, extracted from a royal jelly by performing steps (A) to (F):
- (A) preparing the royal jelly obtained from honeybees selected from one or more species with genbank identifiers including:
- KM066965.1, KM066964.1, KM066963.1, KM066960.1, KM066951.1, KM066962.1, KM066959.1, KM066957.1, KM066961.1, KM066952.1, KM066955.1, KM066956.1, KM066958.1, KM066953.1, and KM066954.1;
- (B) mixing 0.5 g of the royal jelly with 1 mL of methanol and 1 mL of n-hexane in a 10 mL test tube, shaking the test tube for 2 minutes, and subjecting it to ultrasonic treatment at a frequency of 35 kHz for 30 minutes to obtain a mixed solution;
- (C) centrifuging the mixed solution at 2500 rpm for 15 minutes, then filtering to obtain a filtered solution;
- (D) centrifuging the filtered solution for 5 minutes at 4500 rpm to obtain a first solution;
- (E) mixing the first solution with 1 mL of n-hexane and another 1 mL of n-hexane in a 10 mL test tube, shaking the test tube for 2 minutes, and subjecting it to ultrasonic treatment at a frequency of 35 kHz for 30 minutes, repeating step (E) twice to obtain a second solution;
- and
- (F) concentrating the second solution using inert gas nitrogen to obtain the coenzyme Q10 component;
- (k) a ginsenoside Rb1 extract component having a predetermined eleventh percentage (%) by weight, prepared by performing steps (a3) to (g3), including:
- (a3) preparing material:
- obtaining a Panax notoginseng roots aged 3 to 7 years, washing them clean, cutting into small pieces, and drying to a moisture content of less than 15%;
- (b3) extracting the material with 60% ethanol solvent at a ratio of 1:6 w/v using an ultrasonic extraction device at 60° C. for 2 hours, repeating step (b3) three times to obtain a fourth temporary extract;
- (c3) filtering the fourth temporary extract and removing the solvent to obtain an extract concentrate;
- (d3) dissolving the extract concentrate in distilled water at a ratio of 1:6 w/v to obtain a fifth temporary extract;
- (e3) adding n-butanol solution and the fifth temporary extract at a ratio of 2:1 v/v to an ultrasonic extraction device at 60° C. for 2 hours, repeating step (e3) three times to obtain a n-butanol fraction and distilling the solvent under reduced pressure to obtain a sixth temporary extract;
- (f3) centrifuging the sixth temporary extract at 5000 rpm for 10 minutes to obtain a precipitate;
- and
- (g3) drying the precipitate at 75° C. for 3-8 hours to obtain the ginsenoside Rb1 extract component;
- (l) the remainder is a honey component having a predetermined twelfth percentage (%) by weight;
- wherein the total weight percentage from the first to the twelfth components equals 100% by weight;
- (ii) creating a base mixture by heating the honey component at 60° C.-65° C., stirring at 300-500 rpm for 30 minutes;
- (iii) creating a foundation mixture by homogenizing the rice bran oil with the base mixture at step (ii), stirring at 300-500 rpm, and heating for an additional 2-5 minutes;
- (iv) mixing the components (a) to (g) in order with the foundation mixture at step (iii) to create a first temporary mixture, ensuring homogeneous mixing after each addition;
- (v) mixing the legume powder component with the first temporary mixture at step (iv) at 300-500 rpm for 20 minutes to create a second temporary mixture;
- (vi) mixing the coenzyme Q10 component with the second temporary mixture at step (v) at 300-500 rpm for 20 minutes to obtain a uniform mixture;
- (vii) incubating the uniform mixture at 25° C.-37° C. for 10-15 days, adding the ginsenoside Rb1 extract component, and continuing incubation at 25° C.-37° C. for 10-15 days to obtain a post-incubation mixture;
- and
- (viii) concentrating the post-incubation mixture at 50° C. to obtain the plant-based extract composition having the effect of enhancing nutrition and immunity.