Automated Raman signal collection device
Assignee14336186 Canada Corp. (Exclaro)
InventorsRajeev Agarwal, Jean-Phillipe Tremblay, Prashanth Venkataswamy, Richard Nguyen Phat The
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Abstract
A system for automated collection of Raman spectra from ex-vivo tissue samples is provided. The system may comprise a cartesian robot that moves a detachable Raman probe to points on the ex-vivo tissue sample that have been predetermined through a 3D image of the sample. Accordingly, methods of rapid acquisition of multiple Raman spectra from a heterogeneous tissue sample are provided. The inventive methods consider variable contours of the sample to provide Raman spectra from multiple points while generating optimal coverage of the sample. The inventive methods include cleaning the Raman probe between acquisitions of Raman spectra from the multiple points.
Claims13
A system for automated spectroscopy measurements of ex vivo tissue comprising:
- a cartesian robot configured to move along at least three axes;
- a spectroscopic imaging device having a proximal end and a distal end with a tip, detachably connected to the cartesian robot;
- a high-resolution 3D imaging system;
- a spectrometer;
- and
- a stage configured to be positioned under a tissue sample,
- a pressure sensor configured to detect contact of the tip of the spectroscopic imaging device with the tissue sample and to provide feedback information to dynamically adjust a position of the spectroscopic imaging device,
- whereby the cartesian robot is configured to move the spectroscopic imaging device to at least one point on the tissue sample defined by intersections of the at least three axes and based upon tissue sample contour data in an image acquired by the 3D imaging system and according to the feedback information provided by the pressure sensor;
- and
- whereby the spectroscopic imaging device is configured to acquire spectroscopic imaging data at the at least two points.
A method of obtaining automated spectroscopy measurements of ex vivo tissue comprising:
- positioning a tissue sample on a stage of a measurement system comprising:
- a cartesian robot configured to move along at least three axes;
- a spectroscopic imaging device having a proximal end and a distal end with a tip, detachably connected to the cartesian robot;
- a high-resolution 3D camera and spectrometer;
- and
- a stage configured to be positioned under the tissue sample,
- a pressure sensor configured to detect contact of the tip of the spectroscopic imaging device with the tissue sample and to provide feedback information to dynamically adjust a position of the spectroscopic imaging device,
- whereby the cartesian robot is configured to move the spectroscopic imaging device to at least two points on the tissue sample defined by intersections of the at least three axes and based upon tissue sample contour data in an image acquired by the 3D camera and according to the feedback information provided by the pressure sensor;
- and
- whereby the spectroscopic imaging device is configured to acquire spectroscopic imaging data at the at least two points,
- acquiring an image of the tissue sample with the 3D camera;
- providing the 3D camera image to the cartesian robot;
- causing the cartesian robot to move the spectroscopic imaging device to the at least two points on the tissue sample defined by intersections of the at least three axes and based upon the tissue sample contour data in the 3D camera image;
- and
- causing the spectroscopic imaging device to acquire spectroscopic imaging data at the at least two points.
Dependencies are inferred from the claim text.