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US 12,398,409 B2Granted
Yeast cells genetically modified for downregulation of pyruvate decarboxylase activity and FBP-sensitive pyruvate kinase
AssigneeMelt & Marble AB
InventorsAnastasia Krivoruchko, Jens Nielsen, Florian David, Tao Yu
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Abstract
A fungal cell capable of producing high levels of fatty acids and fatty acid-derived products comprises at least one modification to the endogenous fatty acid metabolism.
Claims13
A yeast cell, wherein
- pyruvate decarboxylase activity in said yeast cell is downregulated by the deletion of at least one gene selected from the group consisting of PDC1, PDC5 and PDC6;
- and
- said yeast cell is further genetically modified for
- downregulation of an endogenous fructose-1,6-bisphosphate (FBP)-sensitive pyruvate kinase PYK1;
- and/or
- overexpression of an endogenous fructose-1,6-bisphosphate (FBP)-insensitive pyruvate kinase PYK2, wherein
- said yeast cell has reduced ethanol formation compared to an unmodified yeast cell by said deletion of at least one gene selected from the group consisting of PDC1, PDC5 and PDC6;
- and
- said yeast cell is able to grow on glucose as a carbon source by said downregulation of said endogenous PYK1 and/or said overexpression of said endogenous PYK2.
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Dependencies are inferred from the claim text.