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US 12,398,407 B2Granted
Yeast expressing a heterologous phospholipase for ethanol production
AssigneeNovozymes A/S
InventorsChee-Leong Soong, James Ron Huffman, Monica Tassone, Jung Yi, Hanlin Ouyang
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Abstract
Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
Claims20
A recombinant Saccharomyces yeast cell comprising a heterologous polynucleotide encoding a phospholipase and a heterologous polynucleotide encoding a glucoamylase,
- wherein the phospholipase has a mature polypeptide sequence at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 236, and
- wherein the cell is capable of higher ethanol yield and/or reduced foam accumulation when compared to fermentation using the same process and an identical cell without the heterologous polynucleotide encoding the phospholipase under the same conditions.
Dependencies are inferred from the claim text.