US 12,398,399 B2 · Shoot regeneration by overexpression of CHK genes

Abstract

Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.

Claims17

Dependent claims

A method for improving cytokinin-induced de novo shoot formation of a plant cell, comprising:

(a) increasing or introducing expression of at least one a histidine kinase in the plant cell;
(b) inducing de novo formation of a number of shoots and elongation of the number of shoots by exposing the plant cell of (a) to a cytokinin-comprising medium;
(c) dissecting a de novo formed shoot;
and
(d) inducing root formation of the dissected shoot, wherein the number of de novo formed shoots is increased as compared to an identical cell not having an increased or introduced expression of the histidine kinase, and wherein the histidine kinase has at least 80% sequence identity with SEQ ID NO:
31.

3 more elements

Dependencies are inferred from the claim text.

Shoot regeneration by overexpression of CHK genes

Life of this patent

Shoot regeneration by overexpression of CHK genes

Life of this patent

Abstract

Claims17