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US 12,398,398 B2Granted
Recombinant yeast expressing rubisco and phosphoribulokinase
AssigneeDanisco US Inc.
InventorsAntonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Victor Gabriel Guadalupe Medina, Hendrik Wouter Wisselink
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Abstract
The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.
Claims28
A recombinant yeast cell comprising:
- one or more recombinant heterologous, nucleic acid sequences encoding Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK), wherein the amino acid sequence of the Rubisco has at least 80% sequence identity with the sequence of SEQ ID NO:
- 2, and
- the amino acid sequence of the PRK has at least 70% sequence identity with the sequence of SEO ID NO:
- 4;
- and
- nucleic acid sequences encoding a GroEL
- or a functional homologue of GroEL, and a GroES or a functional homologue of GroES, wherein
- the respective GroEL and GroES homologues are functional as molecular chaperones,
- wherein the amino acid sequence of the GroEL or the functional homologue of GroEL has at least 70% sequence identity with the sequence of SEO ID NO:
- 10;
- and
- wherein the amino acid sequence of the GroES or the functional homologue of GroES has at least 70% sequence identity with the sequence of SEO ID NO:
- 12.
10 more elements
Dependencies are inferred from the claim text.