Inducible promoters
AssigneeWorcester Polytechnic Institute
InventorsAndrés Felipe Carrillo Rincón, Natalie G. Farny
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Abstract
Provided herein are nucleic acid constructs that comprise an inducible promoter. Dual expression systems are provided comprising two nucleic acid constructs or a single nucleic acid construct with two inducible promoters. Also provided are methods of expressing transcripts by transforming a nucleic acid construct described herein into a prokaryotic cell and contacting the prokaryotic cell with an inducer. Methods of producing a carotenoid are also disclosed herein.
Claims16
A nucleic acid construct comprising a modified inducible promoter, wherein the modified inducible promoter comprises:
- a. a TATAATGT sequence at position −10, relative to a transcriptional start site of the promoter;
- b. a TTGACA sequence at position −35, relative to the transcriptional start site of the promoter;
- and
- c. a nucleic acid sequence that encodes a bacterial ribosome binding sequence.
A method of expressing a protein encoded by a transgene in a cell, the method comprising:
- a. transforming a cell with a nucleic acid construct, wherein the nucleic acid construct comprises a modified inducible promoter that comprises:
- i. a TATAATGT sequence at position −10, relative to a transcriptional start site of the promoter;
- ii. a TTGACA sequence at −35 position, relative to the transcriptional start site of the promoter;
- iii. a nucleic acid that encodes a bacterial ribosome binding sequence;
- and
- iv. a transgene;
- and
- b. contacting the cell with an inducer, thereby expressing the protein encoded by the transgene.
Dependencies are inferred from the claim text.