Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol

A non-naturally occurring microbial organism having:

• (i) a formaldehyde fixation pathway;
• and
• (ii) a methanol metabolic pathway, and
• a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway,
• wherein said formaldehyde fixation pathway comprises a 3-hexulose-6-phosphate synthase and a 6-phospho-3-hexuloisomerase
• wherein said methanol metabolic pathway comprises a methanol dehydrogenase,
• wherein said FAACPE cycle comprises one or more β-ketoacyl-ACP synthase, one or more β-ketoacyl-ACP reductase, one or more β-hydroxyacyl-ACP reductase, and one or more enoyl ACP-reductase,
• wherein said termination pathway comprises a thioesterase
• wherein an enzyme of the formaldehyde fixation pathway, the methanol metabolic pathway, the FAACPE cycle or the termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):
• wherein R1 is C1-24 linear alkyl;
• R2 is H;
• and represents a single,
• wherein the substrate of each of said enzymes of the FAACPE cycle and the termination pathway are independently selected from a compound of Formula (II) or malonyl-ACP:
• wherein R1 is C1-24 linear alkyl;
• R3 is H, OH, or oxo (═O));
• R4 is S-CoA, ACP, OH or H;
• and represents a single bond with the proviso that the valency of the carbon atom to which R3 is attached is four,
• wherein said one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R1 that is no greater than the number of carbon atoms at R1 of said compound of Formula (I), and
• wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R1 that is no less than the number of carbon atoms at R1 of said compound of Formula (I).