Back to results
US 12,398,387 B2Granted
Compositions and methods for preparing factor Xa and derivatives
AssigneeAlexion Pharmaceuticals, Inc.
InventorGenmin Lu
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to protein sequences which can be used to generate factor Xa proteins and derivatives thereof. The protein sequences include a factor Xa light chain portion, a heavy chain catalytic domain portion, and an activation peptide C-terminal to the heavy chain catalytic domain portion.
Claims14
A protein comprising the amino acid sequence of formula (I):
- LC-L1-HC-L2-AP (I),
- wherein:
- LC comprises the amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:13,
- L1 is a peptide linker comprising a protease recognition site,
- HC comprises the amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:11,
- L2 is absent or is a peptide linker that cannot be processed by the protease recognizing the protease recognition site in L1, and
- AP is an activation peptide comprising the amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 12;
- and
- wherein, when the L1 is processed by the protease, a two-chain polypeptide comprising the LC and the HC on separate chains is produced, and the two-chain polypeptide binds to a factor Xa (fXa) inhibitor.
7 more elements
Dependencies are inferred from the claim text.