Enzyme variants with improved ester synthase properties

A variant ester synthase polypeptide comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:

• 67, wherein said variant ester synthase polypeptide is genetically engineered to have at least one mutation selected from the group consisting of to K1N, K1T, R2S, G4R, T5P, T5S, L6Q, D7N, S15G, T24M, T24N, T24W, Q26P, Q26T, L30H, G33D, G33N, G33S, L39A, L39M, L39S, R40S, D41A, D41G, D41H, D41Y, V43K, V43S, T44A, T44F, T44K, E48A, E48Q, A49D, A49T, G50S, Y58C, Y58L, V69L, V69W, I70L, I70V, A73G, A73Q, A73S, A73T, V76L, D77A, K78F, K78W, D79H, D79K, I80V, R87W, R93T, R98D, E99Q, G101L, I102L, I102R, S105G, N110R, P111D, P111G, P111S, D113A, D113V, S115R, C121S, H122Q, H122S, V123I, V123M, G126D, L127G, R131M, L134T, T136G, I146K, I146L, I146R, S147A, S147T, V149L, R150P, V155G, T157S, T158E, T158K, T158R, E161D, E161G, R162E, R162K, C163I, C163R, N164D, N164R, M165K, P166L, P166S, T170M, T170R, T170S, V171E, V171F, V171H, V171L, V171R, V171W, R172S, R172W, P173R, P173S, P173W, H174E, Q175E, Q175R, Q175S, R176T, R177V, A179K, A179S, A179V, D182G, K183S, E184F, E184G, E184L, E184R, E184S, A185L, A185M, S186T, V187G, V187R, P188R, A189G, A190P, A190R, A190W, V191I, V191L, S192A, S192L, S192V, Q193R, Q193S, M195G, D196E, A197T, A197V, L200R, Q201A, Q201R, Q201V, Q201W, A202L, D203E, D203R, P206F, R207A, G212A, G212L, G212M, G212S, V216I, V219L, A228G, T231S, V234C, V236A, V236K, L237I, H239G, T242K, T242R, A243G, A243R, Q244G, R246A, R246G, R246L, R246Q, R246P, R246V, R246W, Q250W, Q253G, L254R, L254T, D255E, L257I, L257M, K258R, N259A, N259E, N259Q, L260M, L260V, H262Q, H262R, A263V, S264D, S264V, S264W, G265D, G265N, G266A, G266S, S267G, L268F, L268M, V272A, Y274G, A279G, A279V, A285L, A285R, A285V, E286H, Q287S, N288D, N289E, N289G, D292F, T293A, T293I, P294G, V301A, N302G, I303G, I303R, I303W, R304W, A306G, A306S, D307F, D307G, D307L, D307N, D307R, D307V, E309A, E309G, E309S, G310H, G310R, G310V, T311S, T313S, Q314G, Q314R, I315F, S316G, F317W, I319G, A320C, A323G, A327I, D328F, N331G, N331K, N331T, Q334K, Q334S, Q335C, Q335N, Q335S, T338A, T338E, T338H, Q348A, Q348R, K349A, K349C, K349H, K349Q, P351G, K352I, K352N, S353K, S353T, T356G, T356W, Q357V, M360Q, M360R, M360S, M360W, M363W, P365G, Y366G, Y366W, 1367M, M371R, G373R, G375A, G375S, G375V, M378A, V381F, T385G, E393G, E393Q, E393R, E393T, E393W, G394E, G394R, T395E, T395V, R402K, E404K, E404R, V409L, S410G, L411A, L411P, L411V, A413T, 1420V, C422G, S424G, S424Q, A426V, N430G, S442E, S442G, M443G, A447C, A447I, A447L, L454V, D455E, L457Y, E458G, E458W, I461G, I461L, I461V, K466N, R467L, R467T, A468G, A468S, A468T, R469L, T470A, T470P, T470S, K472T, K472*, *474Y, and a combination thereof, and wherein said variant ester synthase polypeptide has improved ester synthase activity compared to a corresponding wild type polypeptide.