US 12,398,374 B2 · Method for preparing induced pluripotent stem cells by reprogramming somatic cells

Abstract

The present invention provides a method for preparing induced pluripotent stem cells through somatic cell reprogramming and induced pluripotent stem cells obtained therefrom. The present method comprises introducing the factors Oct4 and Nanog as reprogramming-inducing factors into somatic cells to perform reprogramming; followed by culturing the partially or fully reprogrammed somatic cells in a medium comprising specific chemical inducing agents to obtain induced pluripotent stem cells. In the present invention, the combination of different forms of reprogramming-inducing factors and three small-molecule compounds as chemical inducing agents can significantly improve the reprogramming efficiency of human somatic cells and reduce the tumorigenicity of the obtained induced pluripotent stem cells.

Claims15

Dependent claims

A method of generating mammalian induced pluripotent stem cells (iPSCs), the method comprising:

(1) introducing one or more nucleic acids encoding the reprogramming-inducing factors Oct4 and Nanog into mammalian fibroblasts or mammalian erythroid progenitor cells to obtain reprogrammed fibroblasts or erythroid progenitor cells;
(2) culturing the reprogrammed fibroblasts or erythroid progenitor cells obtained in step (1) in the presence of chemical inducing agents to obtain induced pluripotent stem cells (iPSCs), wherein the chemical inducing agents are consisting of a TGFB receptor inhibitor, a cyclic AMP (cAMP) agonist, and a glycogen synthase kinase (GSK) inhibitor,
wherein only Oct4 and Nanog are used as reprogramming-inducing factors.

Dependencies are inferred from the claim text.

Facts

Application

17/597,970Filed 31 Jul 2020

Priority

CN 201910707125.01 Aug 2019

Published

US 2022/0325248 A113 Oct 2022

Granted

26 Aug 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification22 codes

C12N 5/0696
Vertebrate cells: artificially induced pluripotent stem cells, e.g. iPS
C07K 14/4705
Regulators; Modulating activity: stimulating, promoting or activating activity
C12N 5/10
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: cells…
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells

Method for preparing induced pluripotent stem cells by reprogramming somatic cells

Life of this patent

Method for preparing induced pluripotent stem cells by reprogramming somatic cells

Life of this patent

Abstract

Claims15