Culture medium and method for inducing iPSC differentiation to obtain macrophages and use thereof

A kit for inducing induced Pluripotent Stem Cells (iPSC) differentiation to obtain macrophages, comprising:

• a first stage medium, a second stage medium, a third stage medium, a fourth stage medium, a fifth stage medium, and a sixth stage medium;
• wherein
• the first stage medium is an iPSC culture medium containing a ROCK pathway inhibitor and polyvinyl alcohol, the second stage medium is an iPSC culture medium containing a GSK-3B inhibitor, the third stage medium comprises an M1 medium and an M2 medium, the fourth stage medium is an M3 medium, the fifth stage medium is an M4 medium, and the sixth stage medium is an M5 medium;
• the M1 medium comprises a serum-free hematopoietic cells culture medium, DMEM/F12, L-glutamine, ascorbic acid, Insulin-Transferrin-Selenium-Ethanolamine, BMP4, VEGF, and bFGF;
• the M2 medium comprises the M1 medium and inhibitors of TGF—B type I receptors ALK5, ALK4, and ALK7;
• the M3 medium comprises macrophage medium, L-glutamine, ascorbic acid, Insulin-Transferrin-Selenium-Ethanolamine, non-essential amino acids (NEAA), β-mercaptoethanol, BMP4, VEGF, bFGF, SCF, IL-3, and M-CSF;
• the M4 medium comprises macrophage medium, L-glutamine, ascorbic acid, Insulin-Transferrin-Selenium-Ethanolamine, NEAA, β-mercaptoethanol, IL-3, and M-CSF;
• the M5 medium comprises macrophage medium, L-glutamine, ascorbic acid, Insulin-Transferrin-Selenium-Ethanolamine, NEAA, β-mercaptoethanol, and M-CSF;
• the ROCK pathway inhibitor in the first stage medium is selected from the group consisting of Y-27632, Thiazovivin, Fasudil (HA-1077) HCl, GSK429286A, RKI-1447, and Azaindole 1;
• the GSK-3β inhibitor in the second stage medium is selected from the group consisting of CHIR-99021, SB216763, CHIR-98014, TWS119, Tideglusib, and SB415286;
• and
• the inhibitors of the TGF-β type I receptors ALK5, ALK4, and ALK7 in the M2 medium is selected from the group consisting of SB431542, Galunisertib (LY2157299), LY2109761, SB525334, SB505124, and GW788388.