Purified double negative T cell and preparation and use thereof
AssigneeBeijing Immutech LLC
InventorsDong Zhang, Yanbing Zhu, Lu Yang, Dan Tian, Song Wang, Guangyong Sun
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Abstract
Provided is a DNT cell population with IL-17 secretory cells removed and a preparation method therefor and the use thereof. The DNT cell population has the effects of inhibiting T cell proliferation and inducing apoptosis, can inhibit the inflammation of adipose tissue, improve insulin resistance, alleviate diabetes, and relieve steatohepatitis, and can also be used for preventing and treating antigen-specific allergic asthma.
Claims13
A cell preparation comprising a CD4−CD8− Double Negative T cell (DNT cell) population, wherein in the CD4−CD8− DNT cell population more than 95% of the total number of cells do not secrete IL-17;
- wherein the cell preparation is prepared through a method comprising the steps of:
- (a) providing a peripheral blood mononuclear cell population (PBMC cell population), and sorting the PBMC cell population, thereby obtaining a first cell subpopulation dominated by CD4− CD8− DNT cells;
- (b) removing IL-17 secreting DNT cells from the first cell subpopulation, expanding the remained first cell subpopulation, thereby obtaining a second cell subpopulation;
- (c) optionally, further removing IL-17 secreting DNT cells from the second cell subpopulation thereby obtaining a third cell subpopulation;
- (d) mixing the second or the third cell subpopulation with a carrier, thereby obtaining the cell preparation;
- wherein, in step (b), the IL-17 producing DNT cells are removed before expansion by using one or more antibodies targeting an expression protein selected from the group consisting of S100a6, CXCR6, ICOS, CCR2, CD82, CD127, Ltb4r and a combination thereof.
Dependencies are inferred from the claim text.