Methods and compositions for delivery of agents across the blood-brain barrier
AssigneeThe Brigham and Women's Hospital, Inc.
InventorFengfeng Bei
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Abstract
The present invention is based on the development of artificial targeting sequences that enhance permeation of agents into cells and across the blood brain barrier, compositions comprising the sequences, and methods of use thereof. Provided herein is an AAV comprising a capsid protein comprising a targeting sequence and a transgene, preferably a therapeutic or diagnostic transgene. Further, provided herein are methods of delivering a transgene to a cell, the method comprising contacting the cell with an AAV or fusion protein described herein.
Claims11
An AAV capsid protein comprising a targeting sequence that comprises TVSALFK (SEQ ID NO:8);
- TVSALK (SEQ ID NO:4);
- KLASVT (SEQ ID NO:83);
- or KFLASVT (SEQ ID NO:84), wherein the targeting sequence is inserted in a position corresponding to amino acids 588 and 589 of SEQ ID NO:85.
Dependencies are inferred from the claim text.