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US 12,397,283 B1Granted
Chromatography media and methods for producing them
AssigneeCytiva BioProcess R&D AB
InventorsMarc Hummersone, Benjamin Wallis, Frederic Leroux, William Howard Albert Law
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Abstract
The invention discloses a functionalised chromatography medium, comprising: i) at least one non-woven layer of polymeric nanofibres comprising a plurality of nanofibre-nanofibre fusion points; ii) a grafted polymer coating covering the polymeric nanofibres and the nanofibre-nanofibre fusion points; iii) a plurality of ligand groups covalently bound to the grafted polymer coating, wherein the ligand groups are capable of interacting with a target biomolecule.
Claims20
A functionalised chromatography medium, comprising:
- i) at least one non-woven layer of polymeric nanofibres comprising a plurality of nanofibre-nanofibre fusion points;
- ii) a plurality of grafted polymer molecules single-point covalently tethered to said polymeric nanofibers, wherein the plurality of grafted polymer molecules are formed from a plurality of compounds of formula
- and
- iii) a plurality of ligand groups covalently bound to said grafted polymer molecules, wherein the plurality of ligand groups comprise weak or strong anion exchange ligand groups, weak or strong cation exchange ligand groups, hydrophobic interaction chromatography ligand groups, mixed mode chromatography ligand groups, or affinity ligand groups.
1 more element
A functionalised chromatography medium, comprising:
- i) at least one non-woven layer of polymeric nanofibres comprising a plurality of nanofibre-nanofibre fusion points;
- ii) a grafted polymer coating covering said polymeric nanofibres and said nanofibre-nanofibre fusion points, wherein the grafted polymer coating comprises polymer molecules which are single-point covalently tethered to said polymeric nanofibres, and wherein the polymer molecules comprise glycidol monomer residues formed from a plurality of compounds of formula
- and
- iii) a plurality of ligand groups covalently bound to said grafted polymer coating, wherein the plurality of ligand groups comprise weak or strong anion exchange ligand groups, weak or strong cation exchange ligand groups, hydrophobic interaction chromatography ligand groups, mixed mode chromatography ligand groups, or affinity ligand groups.
1 more element
Dependencies are inferred from the claim text.