Delivery of gene therapy treatments
AssigneesIndiana University Research and Technology Corporation, The United States Government as represented by the Department of Veterans Affairs
InventorsRobert Bacallao, Simon Atkinson, George J. Rhodes, Peter Corridon
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Abstract
The present invention provides methods and materials useful delivering liquids, including liquids comprising nucleic acid molecules into cells. In particular, the present invention provides methods for delivering saline solution, exogenous compositions, and isolated vectors to kidney cells, using the renal vein as a guide and under hydrodynamic pressure. The delivery methods and materials herein are useful to research, prognose, ameliorate symptoms of kidney injury, and treat kidney pathologies.
Claims20
A method of delivering a gene therapy treatment for a chronic kidney disease, the method comprising:
- occluding the renal vein to a kidney having the chronic kidney disease;
- administering a retrograde hydrodynamic injection of a therapeutic fluid into the occluded renal vein at a site between the occlusion and the kidney, the retrograde hydrodynamic injection increasing the renal venous pressure during the injection, the therapeutic fluid comprising a nucleic acid molecule;
- waiting, after the retrograde hydrodynamic injection, for a decrease in the renal venous pressure before unoccluding the renal vein;
- and
- unoccluding the renal vein.
A method of delivering a gene therapy treatment for a chronic kidney disease, the method comprising:
- occluding the renal vein to a kidney having the chronic kidney disease;
- administering a retrograde hydrodynamic injection of a therapeutic fluid into the occluded renal vein at a site between the occlusion and the kidney, the retrograde hydrodynamic injection increasing the renal venous pressure during the injection, the therapeutic fluid comprising a nucleic acid molecule encoded by a vector;
- waiting, after the retrograde hydrodynamic injection, for a decrease in the renal venous pressure before unoccluding the renal vein;
- and
- unoccluding the renal vein.
A method of delivering a gene therapy treatment for a chronic kidney disease, the method comprising:
- occluding the renal vein to a kidney having the chronic kidney disease;
- administering a retrograde hydrodynamic injection of a therapeutic fluid into the occluded renal vein at a site between the occlusion and the kidney, the retrograde hydrodynamic injection increasing the renal venous pressure during the injection, the therapeutic fluid comprising a nucleic acid molecule;
- waiting, after the retrograde hydrodynamic injection, for a decrease in the renal venous pressure before unoccluding the renal vein;
- and
- unoccluding the renal vein.
Dependencies are inferred from the claim text.