Methods and kits for classifying cannabinoid production in cannabis plants
AssigneeMedicinal Genomics Corporation
InventorKevin McKernan
Life of this patent
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Abstract
The present disclosure provides methods and kits for characterizing Cannabis plants. Methods and kits of the present disclosure include detection/amplification of one or more enzymes involved in the production of cannabinoids, such as, for example, tetrahydrocannabinolic acid (THCA) synthase and/or cannabidiolic acid (CBDA) synthase.
Claims20
A method of detecting whether a tetrahydrocannabinolic acid (THCA) synthase gene sequence is present in a Cannabis plant, comprising:
- obtaining a sample collected from the Cannabis plant that contains nucleic acids;
- contacting the nucleic acids with at least five primers specific for the THCA synthase gene sequence;
- amplifying the THCA synthase gene sequence when present among the nucleic acids;
- and
- detecting amplicons upon amplification of the THCA synthase gene sequence, wherein:
- the primers specific for the THCA synthase gene sequence include a primer specific for a promoter sequence of the THCA synthase gene sequence;
- amplification of the THCA synthase gene sequence indicates that the THCA synthase gene sequence is at least 2000 nucleotides long;
- and
- a colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay is performed.
A method of detecting whether a tetrahydrocannabinolic acid (THCA) synthase gene sequence is present in a Cannabis plant, comprising:
- obtaining a sample collected from the Cannabis plant that contains nucleic acids;
- contacting the nucleic acids with at least five primers specific for the THCA synthase gene sequence;
- amplifying the THCA synthase gene sequence when present among the nucleic acids;
- and
- detecting amplicons upon amplification of the THCA synthase gene sequence, wherein:
- the primers specific for the THCA synthase gene sequence include a primer specific for a promoter sequence of the THCA synthase gene sequence;
- the primer specific for the promoter sequence of the THCA synthase gene sequence binds to the promoter sequence at a location that is 200-1000 nucleotides upstream of a THCA synthase open reading frame;
- and
- a colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay is performed.
A method of detecting whether a tetrahydrocannabinolic acid (THCA) synthase gene sequence is present in a Cannabis plant, comprising:
- obtaining a sample collected from the Cannabis plant that contains nucleic acids;
- contacting the nucleic acids with at least five primers specific for the THCA synthase gene sequence;
- amplifying the THCA synthase gene sequence when present among the nucleic acids;
- and
- detecting amplicons upon amplification of the THCA synthase gene sequence, wherein:
- the primers specific for the THCA synthase gene sequence include a primer specific for a promoter sequence of the THCA synthase gene sequence;
- the amplicons of the THCA synthase gene sequence include at least part of the THCA synthase promoter and 5′ UTR;
- and
- a colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay is performed.
Dependencies are inferred from the claim text.