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US 12,391,981 B2Granted
Chromogenic peroxidase substrates
AssigneeAgilent Technologies, Inc.
InventorsJesper Lohse, Mike Paw Maischnack Hansen
Life of this patent
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Abstract
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
Claims21
A composition comprising:
- (a) a mixture of at least a first chromogen and at least a second chromogen, wherein said chromogens are mixed in a ratio which produces a desired color and wherein said first chromogen comprises
- (a) a chromogenic moiety, and
- (b) a peroxidase substrate moiety,
- wherein the chromogenic moiety and the peroxidase substrate moiety are linked together via a linker to form a conjugate,
- wherein the conjugate is a compound of Formula I:
- where X is —OH, —ORX or —NRXRXX,
- where Y is ═O, ═NRY, or ═N+RYRYY,
- wherein R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, RX, RXX, RY, and RYY are independently selected from hydrogen and a substituent having less than 40 atoms;
- L is a linker comprising a linear chain of 5 to 29 consecutively connected atoms;
- and
- PS is a peroxidase substrate moiety.
8 more elements
Dependencies are inferred from the claim text.