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US 12,391,974 B2Granted
Methods of detecting alloantibodies to HLA class II antigens
AssigneesEmory University, One Lambda
InventorsRobert A. Bray, Jar-How Lee
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Abstract
Described herein are materials and methods of incorporating CLIP peptide into the peptide binding groove of HLA Class II antigens and using such HLA Class II antigens for the detection of alloantibodies.
Claims12
A method of screening for antibodies that specifically bind an HLA Class II antigen in which a CLIP peptide is present in the peptide binding groove comprising the steps of:
- preparing a panel comprising a solid-phase substrate having an HLA Class II antigen in which a CLIP peptide is present in the peptide binding groove immobilized or attached thereto, wherein the CLIP peptide is a subsequence of human CD74 invariant chain comprising MRMATPLLM (SEQ ID NO:
- 1);
- obtaining a serum sample from a human subject,
- contacting the panel with the serum sample, and
- detecting binding of antibodies in said serum sample to the HLA Class II antigen in which a CLIP peptide is present in the peptide binding groove.
2 more elements
Dependencies are inferred from the claim text.