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AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsShota Tanaka, Atsushi Ohta, Tetsuo Kojima
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An objective of the present invention is to provide methods of synthesizing peptides containing structurally diverse amino acids using cell-free translation systems, which can accomplish excellent translational efficiency as compared to conventional techniques (the conventional techniques being methods which involve preparing aminoacyl-tRNAs which do not have protecting groups outside the translation systems without using ARS, and then adding the prepared aminoacyl-tRNAs into translation systems). In the present invention, it was found that amino acid-containing peptides can be synthesized efficiently by protecting an amino acid linked to tRNA with an appropriate protecting group, and then performing the step of deprotecting the protecting group of the amino acid linked to tRNA and the step of peptide translation from a template nucleic acid in a cell-free translation system in parallel.
A method of synthesizing a peptide, wherein the method comprises a step of deprotecting a protecting group of an amino acid having the protecting group on the amino group that forms a peptide bond and a translation step, in a cell-free translation system that comprises tRNA to which the amino acid having the protecting group is linked, wherein the deprotecting and translation steps are performed in parallel, wherein the protecting group is
Dependencies are inferred from the claim text.