Biopolymer film and method of preparing the same

A method of producing biopolymer films comprising:

• pre-treating a carbon source to obtain an apple pomace lysate by:
• reconstituting and filtering a waste apple pomace to form a filtrate;
• drying the filtrate for a day at 55-60° C.;
• grinding the dried filtrate to form a powder;
• and
• hydrolyzing the powder with an acid at 100° C. for 15 min to form lysate;
• and
• sterilizing the lysate to form the apple pomace lysate;
• preparing a basal media;
• preparing an inoculum, wherein the inoculum includes a pure culture of Cupriavidus necator, adding the apple pomace lysate and the inoculum in the basal media so as to selectively modify the metabolic pathway of the Cupriavidus necator therein;
• fermenting the apple pomace lysate with the inoculum in the basal media to produce a biopolymer;
• recovering the biopolymer resulting from the step of fermentation;
• and
• blending the recovered biopolymer with at least one blending agent by:
• dissolving the biopolymer and at least one of ethyl cellulose (EtC) or cellulose acetate butyrate (CAB) in a ratio of
• 50 parts of the biopolymer with 50 parts of cellulose acetate butyrate (CAB), or 90 parts of the biopolymer with 10 parts of ethyl cellulose (EtC) in an organic solvent;
• and
• evaporating the organic solvent to produce one or more biopolymer films, wherein the biopolymer film is a mixture of polyhydroxybutyrate (PHB) and a blending agent;
• wherein, the biopolymer film having 90 parts of polyhydroxybutyrate (PHB) with 10 parts ethyl cellulose (EtC), the biopolymer film, by percent thereof, biodegrades at least 71% within 6 months;
• and
• wherein, the biopolymer film having 50 parts of polyhydroxybutyrate (PHB) with 50 parts cellulose acetate butyrate (CAB), the biopolymer film provides elongation at break ranging from 3.876% to 6.552%.