Targeting peptide to deliver a compound to oocytes
AssigneeThe Penn State Research Foundation
InventorsJason Laurence Rasgon, Duverney De Jesus Chaverra Rodriguez, Grant Leslie Hughes, Chan Chin Heu
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Abstract
Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR/Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.
Claims22
A method for delivery of one or more molecules of interest to an oocyte of an animal, the method comprising,
- injecting into a vitellogenic female animal a functional fragment of a yolk protein precursor (YPP) linked to said one or more molecules of interest,
- wherein the YPP is a Drosophila Yolk Protein 1 (YP1) and wherein the functional fragment has 100% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 3 and is capable of targeting the one or more molecules of interest to the oocyte.
A method of producing a gene edited animal having a stably inheritable edited genome, the method comprising,
- a) injecting into a vitellogenic female animal a functional fragment of a yolk protein precursor (YPP) linked to one or more molecules of interest,
- wherein the YPP is a Drosophila Yolk Protein 1 (YP1), and wherein the functional fragment has 100% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 3 and is capable of targeting the one or more molecules of interest to an oocyte, and wherein the one or more molecules of interest comprise at least one molecule of a gene editing system;
- b) delivery of said molecule of interest to said oocyte of said animal under conditions such that said animal genome is edited and stable;
- c) said animal producing progeny that inherit said edited genome.
A method of increasing the number of gene edited mosquitoes, the method comprising,
- a) injecting into one or more vitellogenic female mosquitos of a population a functional fragment of a yolk protein precursor (YPP) linked to one or more molecules of interest,
- wherein the YPP is a Drosophila Yolk Protein 1 (YP1), and wherein the functional fragment has 100% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 3 and is capable of targeting the one or more molecules of interest to an oocyte, and wherein the one or more molecules of interest comprise at least one molecule of a gene editing system;
- b) delivery of said molecule of interest to said oocyte of said mosquito;
- c) said molecule of interest delivered at 30 hours or less post blood meal feeding of said mosquito or delivered with chloroquine in an amount of 0.5 to 1 mM of chloroquine, or both;
- d) editing one or more sequences of said mosquito populations, wherein an increased number of gene edited mosquitoes are produced compared to a method which does not use step a).
Dependencies are inferred from the claim text.