Methods and products for transfecting cells
AssigneeFactor Bioscience Inc.
InventorsMatthew Angel, Christopher Rohde
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Abstract
The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.
Claims20
A method for generating gene-edited cells, the method comprising:
- (a) providing a plurality of cells comprising a target DNA sequence, wherein the plurality of cells is derived from a human subject;
- (b) culturing the plurality of cells under conditions that allow the plurality of cells to proliferate;
- and
- (c) contacting the plurality of cells in vitro or ex vivo with a plurality of synthetic RNA molecules,
- wherein the plurality of synthetic RNA molecules comprises a nucleotide sequence that encodes a transcription activator-like effector nuclease,
- wherein the plurality of synthetic RNA molecules is added to a medium surrounding the plurality of cells, and
- wherein the contacting results in the plurality of cells internalizing the plurality of synthetic RNA molecules and expressing the transcription activator-like effector nuclease to result in a single-strand break or a double-strand break in the target DNA sequence, thereby generating the gene-edited cells.
Dependencies are inferred from the claim text.