Recombinant Newcastle disease viruses and uses thereof for the prevention of RSV disease or human metapneumovirus disease

A recombinant Newcastle disease virus (NDV) comprising a packaged genome comprising

• (a) a transgene encoding a fusion (F) protein of a human virus of the family Pneumoviridae, wherein the transgene comprises
• (i) a ribonucleic acid (RNA) sequence corresponding to the negative sense of the complementary deoxyribonucleic acid (cDNA) sequence of SEQ ID NO:
• 2, 26, 28, or 30, or
• (ii) an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO:
• 18, 53, 55, or 57;
• (b) a transgene encoding a chimeric protein comprising an ectodomain of a F protein of a human virus of the family Pneumoviridae and a transmembrane domain and a cytoplasmic domain of a NDV F protein, wherein:
• (i) the human virus of the family Pneumoviridae is human respiratory syncytial virus (“RSV”), and the chimeric protein comprises the ectodomain of the amino acid sequence set forth in SEQ ID NO:7,
• (ii) the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO:
• 19, 32, 34, 35, or 36, or
• (iii) the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO:
• 4, 44, 45, or 46;
• or
• (c) a transgene encoding a bovine respiratory syncytial virus (“RSV”) F protein, wherein the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO:
• 11, 41, or 43;
• or
• (d) a transgene encoding a chimeric protein comprising a bovine Respiratory Syncytial Virus (RSV) F protein ectodomain and a transmembrane domain and a cytoplasmic domain of a NDV F protein, wherein:
• (i) the chimeric protein comprises the amino acid sequence set forth in SEQ ID NO:33, or
• (ii) the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO:
• 14, 31, 38, or 39.