Methods, compositions and cells for preparing surfactant protein D (SP-D)
AssigneesAirway Therapeutics, Inc., Glycotope GmbH
InventorsJan Susan Rosenbaum, Frederick Gyapon Quast, Matthias Kaup, Lars Stöckl
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Abstract
Some embodiments of the methods and compositions provided herein relate to the preparation surfactant protein-D (SP-D). Some embodiments include the expression of human SP-D in certain cell lines, and the purification of human SP-D from such cell lines. Some embodiments include the preparation of certain oligomeric forms of human SP-D.
Claims18
A method for producing a human surfactant protein D (SP-D) polypeptide composition comprising:
- (a) introducing a polynucleotide encoding the SP-D polypeptide into a human cell selected from the group consisting of NM-H9D8, NM-H9D8-E6Q12, NM-H9D8(8B11), and NM-F9;
- (b) culturing the cell under conditions in which the SP-D polypeptide is expressed;
- and
- (c) isolating the expressed SP-D polypeptide from the cell,
- wherein
- the expressed SP-D polypeptide has an antennarity number within a range from 190 to 215, or
- an antennarity number for the expressed SP-D polypeptide and an antennarity number for a naturally occurring human SP-D are within 10% from each other.
An isolated human cell comprising an expression vector comprising a polynucleotide encoding a human surfactant protein D (SP-D) polypeptide, wherein the cell is selected from the group consisting of NM-H9D8, NM-H9D8-E6Q12, NM-H9D8(8B11), and NM-F9;
- and
- wherein the cell is capable of expressing the SP-D polypeptide having an antennarity number that is within 10% from an antennarity number for a naturally occurring human SP-D, or wherein the an antennarity number is within a range from 190 to 215.
Dependencies are inferred from the claim text.