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US 12,391,948 B2Granted
RNA-regulated fusion proteins and methods of their use
AssigneeCornell University
InventorsSamie R. Jaffrey, Jiahui Wu
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Abstract
The present disclosure is directed to RNA-regulated fusion proteins comprising a protein of interest and an RNA-regulated destabilization domain. Also disclosed are RNA aptamers that bind specifically to a RNA-regulated destabilization domain. Nucleic acid molecules encoding the RNA-regulated fusion proteins and RNA aptamers and methods of use thereof are also disclosed.
Claims10
A nucleic acid molecule encoding an RNA-regulated fusion protein, said nucleic acid molecule comprising:
- a first nucleic acid sequence encoding a protein of interest and a second nucleic acid sequence encoding an RNA-regulated destabilization domain,
- wherein the second nucleic acid sequence is operably coupled to the first nucleic acid sequence,
- wherein the RNA-regulated destabilization domain is a bifunctional peptide comprising:
- a lentiviral transactivator of transcription (Tat) peptide and
- a degron peptide,
- wherein an RNA aptamer interacts with the RNA-regulated destabilization domain to stabilize the protein of interest, and
- wherein the RNA-regulated destabilization domain is tDeg as set forth in SEQ ID NO:
- 63.
5 more elements
Dependencies are inferred from the claim text.