Biosynthesis of products from 1-carbon compounds

A method for the production of a product from a single carbon substrate, the method comprising the steps of:

• a) growing a genetically modified microorganism in a culture comprising the single carbon substrate and a growth media,
• b) converting said single carbon substrate to a formyl-CoA,
• c) condensing an initial C(n)-aldehyde with the formyl-CoA to produce 2-hydroxy-C(n+1)-acyl-CoA, wherein the 2-hydroxy-C(n+1)-acyl-CoA is one carbon longer than the initial C(n) aldehyde and wherein n is the number of carbons in the initial C(n)-aldehyde;
• d) converting the 2-hydroxy-C(n+1)-acyl-CoA to a C(n+1)-aldehyde;
• e) isolating a product from said microorganism, or said growth media, or both;
• wherein the single carbon substrate is selected from the group consisting of methane, methanol, formaldehyde, formate, carbon monoxide, carbon dioxide, and combinations thereof;
• and
• wherein the microorganism expresses:
• i. a 2-hydroxyacyl-CoA lyase or an oxalyl-CoA decarboxylase that catalyzes the condensation of the initial C(n)-aldehyde with the formyl-CoA to produce the 2-hydroxy-C(n+1)-acyl-CoA;
• ii. one or more polypeptides that catalyze the conversion of the single carbon substrate to the formyl-CoA;
• and
• iii. one or more polypeptides that catalyze a conversion of the 2-hydroxy-C(n+1)-acyl-CoA to the C(n+1)-aldehyde.