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US 12,391,935 B2Granted
Compositions comprising digestive enzymes
AssigneeClara Foods Co.
InventorsHalle Redfearn, Harshal Kshirsagar, Kritika Mahadevan, Alexandre Chapeaux, Wesley Rutherford-Jenkins, Joel Andrew Kreps, Isha Joshi
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Abstract
Provided herein are compositions with enhanced protein specific activity, protein combinations and methods for the preparation thereof.
Claims12
A method for producing a consumable composition comprising combining a high-activity stable pepsin powdered composition with one or more consumable ingredients comprising steps of:
- (a) providing a microorganism expressing a recombinant pepsinogen, wherein the expressed pepsinogen is secreted by the microorganism into its growth media;
- (b) harvesting the growth media and removing the microorganismal cells therefrom to obtain a liquid starting material;
- (c) lowering the pH of the liquid starting material to less than pH 4.0 to obtain an activated pepsin composition;
- (d) raising the activated pepsin composition to a pH of 5.4 or higher to obtain a high-activity stable pepsin composition;
- and
- (e) isolating the stable pepsin polypeptide from protein and small molecules in the liquid starting material to obtain the high-activity stable pepsin powdered composition having a specific activity from 500 FCC units/mg total protein to 50,000 FCC units/mg total protein;
- (f) combining the high-activity stable pepsin powdered composition with the one or more consumable ingredients to produce a consumable composition;
- and
- wherein step (e) is performed after steps (c) and (d).
6 more elements
Dependencies are inferred from the claim text.