Platelet lysate production method, production system, and bag set
AssigneeTerumo Kabushiki Kaisha
InventorKaoru Hosoe
Life of this patent
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Abstract
Provided are a method for producing a platelet lysate, a production system, and a bag set capable of efficiently producing a platelet lysate with a simple device structure. According to the method for producing a platelet lysate, by which a platelet lysate (PL) that contains a growth factor contained in platelet is produced, buffy coat is collected from whole blood; the buffy coat (BC) is centrifuged to extract a supernatant, thereby collecting a platelet concentrate from which leukocyte has been removed; and, prior to freezing, the platelet concentrate is centrifuged to remove the supernatant, thereby preparing a highly concentrated platelet concentrate. The highly concentrated platelet concentrate is then frozen and thawed, and further centrifuged to recover the platelet lysate.
Claims3
A bag set for recovering platelet from a leukapheresis filter, the bag set comprising:
- a first whole blood bag that pools whole blood of a donor;
- a leukapheresis filter connected to a downstream side of the first whole blood bag;
- a second whole blood bag connected through the leukapheresis filter to the downstream side of the first whole blood bag;
- a recovery liquid bag connected to a downstream side of the leukapheresis filter, and pooling a platelet recovery liquid for recovering platelet from the leukapheresis filter;
- a concentration bag set connected to an upstream side of the leukapheresis filter, wherein the concentration bag set includes a platelet recovery bag and a platelet lysate bag connected through a tube and a filter to the platelet recovery bag;
- and
- a first tube that connects the first whole blood bag to the second whole blood bag through the leukapheresis filter, wherein the first tube includes:
- a first branch that connects to the platelet recovery bag;
- and
- a second branch that connects to the recovery liquid bag.
A bag set for recovering platelet from a leukapheresis filter, the bag set comprising:
- a first whole blood bag that pools whole blood of a donor;
- a leukapheresis filter connected to a downstream side of the first whole blood bag;
- a second whole blood bag connected through the leukapheresis filter to the downstream side of the first whole blood bag;
- a recovery liquid bag connected to an upstream side of the leukapheresis filter, and pooling a rinsing liquid for recovering residual blood from the leukapheresis filter;
- a residual blood recovery bag connected to a downstream side of the leukapheresis filter;
- a recovery bag set connected to the downstream side of the leukapheresis filter through a clamp, wherein the recovery bag set includes the residual blood recovery bag and a separation bag connected through a first tube to the residual blood recovery bag;
- and
- a second tube that connects the first whole blood bag to the second whole blood bag through the leukapheresis filter, wherein the second tube includes:
- a first branch that connects to the recovery liquid bag;
- and
- a second branch that connects to the residual blood recovery bag.
Dependencies are inferred from the claim text.