Media for culturing stem cells
AssigneeTechnion Research & Development Foundation Limited
InventorsMichal Amit, Joseph Itskovitz-Eldor
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Abstract
Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.
Claims17
A method of generating lineage-specific cells from primate embryonic stem cells, the method comprising:
- (a) culturing the primate embryonic stem cells in a protein carrier-free culture medium comprising basic fibroblast growth factor (bFGF) and at least one factor selected from the group consisting of:
- transforming growth factor beta-3 (TGFβ3) isoform, transforming growth factor beta-1 (TGFβ1) isoform and IL6RIL6 chimera, wherein said IL6RIL6 chimera comprises amino acids 112-355 of SEQ ID NO:
- 32,
- to thereby obtain expanded, undifferentiated embryonic stem cells;
- (b) subjecting said expanded, undifferentiated primate embryonic stem cells to culturing conditions suitable for differentiating and/or expanding lineage specific cells;
- thereby generating the lineage-specific cells from the primate embryonic stem cells.
A method of generating embryoid bodies from primate embryonic stem cells, the method comprising:
- (a) culturing the primate embryonic stem cells in a protein carrier-free culture medium comprising basic fibroblast growth factor (bFGF) and at least one factor selected from the group consisting of:
- transforming growth factor beta-3 (TGFβ3) isoform, transforming growth factor beta-1 (TGFβ1) isoform and IL6RIL6 chimera, wherein said IL6RIL6 chimera comprises amino acids 112-355 of SEQ ID NO:
- 32,
- to thereby obtain expanded, undifferentiated embryonic stem cells;
- and
- (b) subjecting said expanded, undifferentiated embryonic stem cells to culturing conditions suitable for differentiating said primate embryonic stem cells to embryoid bodies;
- thereby generating the embryoid bodies from the primate embryonic stem cells.
A method of generating lineage-specific cells from primate embryonic stem cells, the method comprising:
- culturing the primate embryonic stem cells in a protein carrier-free culture medium comprising basic fibroblast growth factor (bFGF) and at least one factor selected from the group consisting of:
- transforming growth factor beta-3 (TGFβ3) isoform, transforming growth factor beta-1 (TGFβ1) isoform and IL6RIL6 chimera, wherein said IL6RIL6 chimera comprises amino acids 112-355 of SEQ ID NO:
- 32, to thereby obtain expanded, undifferentiated primate embryonic stem cells;
- (b) subjecting said expanded, undifferentiated primate embryonic stem cells to culturing conditions suitable for differentiating said expanded, undifferentiated embryonic stem cells to embryoid bodies;
- and
- (c) subjecting cells of said embryoid bodies to culturing conditions suitable for differentiating and/or expanding lineage specific cells;
- thereby generating the lineage-specific cells from the primate embryonic stem cells.
Dependencies are inferred from the claim text.