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US 12,390,775 B1Granted
Devices and methods for reducing the effects of settling of particles during droplet production
Assignee10x Genomics, Inc.
InventorsDaniel Freitas, Brenden Janatpour Brown, Francis Cui, Martin Sauzade
Life of this patent
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Abstract
Devices, methods, and kits for generating droplets are provided. The devices, methods, and kits are designed to reduce the effects of settling of particles during droplet production.
Claims24
A device for producing droplets comprising:
- (a) a first channel having a first height, a first width, a first proximal end, and a first distal end;
- (b) a reservoir having a reservoir height greater than the first height, and an outlet in fluid communication with the first proximal end;
- (c) a platform having a platform height less than the reservoir height and comprising a depression on a top surface, wherein the platform is disposed in the reservoir;
- and
- (d) a droplet source region in fluid communication with the first distal end.
2 more elements
A device for producing droplets comprising:
- (a) a first channel having a first height, a first width, a first proximal end, and a first distal end;
- (b) a reservoir having a reservoir height greater than the first height and an outlet in fluid communication with the first proximal end, wherein the reservoir comprises a slanted wall that asymmetrically reduces the cross-sectional area of the reservoir adjacent to the outlet by between 75-90%, wherein the cross-sectional area of the reservoir adjacent to the outlet does not overlap with the radial axis of symmetry of the cross-sectional area of the reservoir at the reservoir height;
- and
- (c) a droplet source region.
1 more element
A device for producing droplets comprising:
- (a) a first channel having a first height, a first width, a first proximal end, and a first distal end;
- (b) a reservoir having a reservoir height greater than the first height and an outlet in fluid communication with the first proximal end, wherein the reservoir comprises a slanted wall that reduces the cross-sectional area of the reservoir adjacent to the outlet by between 75-90%;
- and
- (c) a droplet source region, wherein the droplet source region comprises a shelf region having a third height and a third width greater than the first width and being in fluid communication with the first distal end;
- and a step region comprising a wall having a fourth height greater than the first and third heights, wherein the shelf region is disposed between the step region and the first distal end.
2 more elements
Dependencies are inferred from the claim text.