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US 12,389,889 B2Granted
Mouse having a humanized cluster of differentiation 47 gene
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsCagan Gurer, Ella Ioffe, Alexander Mujica, Gavin Thurston
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Abstract
Non-human animals, and methods and compositions for making and using the same, are provided, wherein said non-human animals comprise a humanization of an endogenous cluster of differentiation (CD) gene, in particular a humanization of a CD47 gene. Said non-human animals may be described, in some embodiments, as having a genetic modification to an endogenous CD47 gene so that said non-human animals express a CD47 polypeptide that includes a human portion and a non-human portion (e.g., a murine portion).
Claims9
A targeting nucleic acid vector, comprising
- a 5′ homology arm comprising a genomic sequence upstream of exon 2 of a mouse CD47 gene,
- a genomic DNA fragment comprising exons 2-7 of a human CD47 gene,
- a drug selection cassette, and
- a 3′ homology arm comprising a genomic sequence downstream of exon 7 of the mouse CD47 gene,
- wherein the genomic fragment comprising exons 2-7 of the human CD47 gene encodes a polypeptide comprising an amino acid sequence having at least 98% identity with the amino acid sequence as set forth in amino acids 16-292 of SEQ ID NO:
- 10,
- wherein the genomic sequence upstream of exon 2 of the mouse CD47 gene comprises exon 1 of the mouse CD47 gene,
- wherein the genomic sequence downstream of exon 7 of the mouse CD47 gene comprises the exons downstream of exon 7 of the mouse CD47 gene, and
- wherein the 5′ and 3′ homology arms mediate integration of the genomic fragment comprising exons 2-7 of the human CD47 gene into a mouse CD47 locus to form a humanized CD47 gene.
6 more elements
Dependencies are inferred from the claim text.