9-LOX5 gene variant providing powdery mildew resistance
AssigneeRijk Zwaan Zaadteelt En Zaadhandel B.V.
InventorsValentina Bracuto, Emilio Sarria Villada, Adrianus Cornelis Koeken, Sara Movahedi
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Abstract
The present invention relates to a plant with a copy number variation (CNV) of a 9-LOX5 gene, wherein the CNV has at least 2 copies of a 9-LOX5 gene, wherein the 9-LOX5 gene comprises SEQ ID NO: 1, or a sequence having at least 60% sequence identity to SEQ ID NO: 1, and wherein the presence of the CNV leads to powdery mildew resistance in the plant. The invention further relates to nucleic acid molecules, methods for selecting powdery mildew resistant plants, the plants thus obtained therefrom, and seeds and parts thereof.
Claims20
An agronomically elite Citrullus lanatus plant that produces fruits with red flesh comprising a copy number variation (CNV) of a 9-LOX5 gene,
- which CNV comprises at least 2 copies of a 9-LOX5 gene on the same chromosome,
- wherein the 9-LOX5 gene comprises:
- one or more copies of a 9-LOX5_1 allele having the sequence of SEQ ID NO:
- 1 or a sequence having at least 99% sequence identity to SEQ ID NO:
- 1, and/or
- one or more copies of a 9-LOX5_2 allele having the sequence of SEQ ID NO:
- 3 or a sequence having at least 99% sequence identity to SEQ ID NO:
- 3,
- wherein the presence of the CNV leads to resistance to at least Podosphaera xanthii in the agronomically elite plant.
A method for producing an agronomically elite Citrullus lanatus plant that is resistant to at least Podosphaera xanthii and that produces fruits with red flesh, said method comprising:
- introducing into a Citrullus lanatus plant that produces fruits with red flesh a copy number variation (CNV) of a 9-LOX5 gene,
- which CNV comprises at least 2 copies of a 9-LOX5 gene on the same chromosome,
- wherein the 9-LOX5 gene comprises:
- one or more copies of a 9-LOX5_1 allele having the sequence of SEQ ID NO:
- 1 or a sequence having at least 99% sequence identity to SEQ ID NO:
- 1, and/or
- one or more copies of a 9-LOX5_2 allele having the sequence of SEQ ID NO:
- 3 or a sequence having at least 99% sequence identity to SEQ ID NO:
- 3,
- wherein the presence of the CNV leads to resistance to at least Podosphaera xanthii in the agronomically elite plant,
- whereby there is producing of the agronomically elite Citrullus lanatus plant that is resistant to at least Podosphaera xanthii and that produces fruits with red flesh.
A method for selecting a Citrullus lanatus plant that is resistant to at least Podosphaera xanthii and that produces fruits with red flesh comprising:
- determining in a Citrullus lanatus plant that produces fruits with red flesh the presence of a copy number variation (CNV) of a 9-LOX5 gene,
- which CNV comprises at least 2 copies of a 9-LOX5 gene on the same chromosome,
- wherein the 9-LOX5 gene comprises:
- one or more copies of a 9-LOX5_1 allele having the sequence of SEQ ID NO:
- 1 or a sequence having at least 99% sequence identity to SEQ ID NO:
- 1, and/or
- one or more copies of a 9-LOX5_2 allele having the sequence of SEQ ID NO:
- 3 or a sequence having at least 99% sequence identity to SEQ ID NO:
- 3,
- wherein the presence of the CNV leads to resistance to at least Podosphaera xanthii in the plant, and
- selecting the Citrullus lanatus plant that produces fruits with red flesh and that from the determining step comprises said CNV of the 9-LOX5 gene,
- whereby there is selecting of the Citrullus lanatus plant that is resistant to at least Podosphaera xanthii and produces fruits with red flesh.
An agronomically elite Citrullus lanatus seed comprising two or more copies of SEQ ID NO:
- 1 or of a sequence having at least 99% sequence identity thereto, or two or more copies of SEQ ID NO:
- 3 or of a sequence having at least 99% sequence identity thereto.
An agronomically elite Citrullus lanatus seed comprising one or more copies of SEQ ID NO:
- 1 or of a sequence having at least 99% sequence identity thereto, and one or more copies of SEQ ID NO:
- 3 or of a sequence having at least 99% sequence identity thereto.
Dependencies are inferred from the claim text.