Homogeneous enzyme immunoassay for keratinized structures
AssigneePsychemedics Corporation
InventorsGary Neil Stowe, Elvan Loni, Virginia Ann Hill, Michael Irving Schaffer
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Abstract
Methods and kits for determining the presence and/or amount of one or more analytes in a keratinized structure (e.g., hair) sample. Such methods can include combining in an aqueous medium: a conjugate comprising an enzyme and an analyte of interest, an antibody specific for the analyte of interest, and the keratinized structure sample, and then determining whether the analyte of interest is present or absent in the keratinized structure sample.
Claims20
A method of determining an amount of an analyte of interest in a keratinized structure sample at a sensitivity of between 2 ng and 5 ng analyte per about 10 mg sample, comprising:
- (a) combining in an aqueous medium:
- (i) a conjugate comprising an enzyme and the analyte of interest;
- (ii) an antibody specific for the analyte of interest;
- and
- (iii) the keratinized structure sample;
- and
- (b) determining the amount of the analyte of interest in the keratinized structure sample at a sensitivity of between 2 ng and 5 ng analyte per about 10 mg sample.
A method of determining an amount of an analyte of interest in a keratinized structure sample at a sensitivity of between 2 ng and 5 ng analyte per about 10 mg sample, comprising:
- (a) combining in an aqueous medium:
- (i) an enzyme-analyte conjugate comprising glucose-6-phosphate dehydrogenase (G6PDH) covalently linked to the analyte of interest;
- (ii) an antibody specific for the analyte of interest;
- (iii) a keratinized structure sample;
- (iv) an enzyme substrate for G6PDH;
- and
- (v) a coenzyme for G6PDH;
- and
- (b) determining the amount of the analyte of interest in the keratinized structure sample at a sensitivity of between 2 ng and 5 ng analyte per about 10 mg sample.
Dependencies are inferred from the claim text.