Electron microscopy grids and high-resolution structural determination methods

An electron microscopy grid, comprising:

• a perforated substrate;
• a support film on the perforated substrate;
• a mixture of linker molecules, wherein said mixture of linker molecules comprises:
• A. at least a first linker molecule according to Structure (I)
• wherein
• AG is an anchoring group, for anchoring the linker molecule to the support film;
• BU is a binding unit, for binding to the analyte;
• L1 is a first linear linker section;
• L2 is a second linear linker section;
• α is the angle between linear linker section L1 and linear linker section L2;
• AS is an angled linker section, connecting linear linker section L1 and linear linker section L2;
• and,
• B. at least a second linker molecule, wherein the second linker molecule is:
• a. a linker molecule according to structure (I), wherein at least AS, L1, L2 or α is different from the AS, L1, L2 or α of the first linker molecule;
• or,
• b. a linker molecule according to structure (II):
• wherein
• AG is an anchoring group, for anchoring the linker molecule to the support film;
• BU is a binding unit, for binding to the analyte;
• L3 is a third linear linker section;
• wherein the linker molecules are attached to the support film via the anchoring group.