Assays and methods for determining microbial resistance

A kit for identification of one or more β-lactamase genes, wherein the one or more β-lactamase genes are selected from the group consisting of:

• CMY, CTX-M, OXA, IMP, VIM, DHA, KPC, and NDM genes, the kit comprising probes comprising the following sequences:
• ACGCTAACTCCAGCATTGGTCTGT (SEQ ID NO:
• 154), AACTTGCCGAATTAGAGCRGCAGT (SEQ ID NO:
• 157), TATCATTGGTGGTGCCGTAGTCGC (SEQ ID NO:
• 160), TGAGACACGGTCCAGACTCCTACG (SEQ ID NO:
• 163), TAGCTTGATCGCCCTCGATTTGGG (SEQ ID NO:
• 166), TTRTTYGGTGGTTGYTTTRTTAA (SEQ ID NO:
• 169), CCGGACGGTCTTGGTAATTTGGGT (SEQ ID NO:
• 172), CCGTACGGTTTAGGCAATTTGGGT (SEQ ID NO:
• 173), AGCTCTTCTATCCTGGTGCTGCG (SEQ ID NO:
• 176), TGAGACACGGTCCAGACTCCTACG (SEQ ID NO:
• 179), AAACCGGGCGATATGCGTCTGTAT (SEQ ID NO:
• 182), TCGTCGCGGAACCATTCGCTAAA (SEQ ID NO:
• 185), CATCAGGACAAGATGGGCGGTATG (SEQ ID NO:
• 188), and TGAGACACGGTCCAGACTCCTACG (SEQ ID NO:
• 191), wherein each probe comprises a fluorophore and/or a fluorescent quencher.