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US 12,385,088 B2Granted
Linear double stranded DNA coupled to a single support or a tag and methods for producing said linear double stranded DNA
AssigneeCureVac AG
InventorsBenyamin Yazdan Panah, Tilmann Roos, Veronika Wagner, Carola Pongratz
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Abstract
The present invention is concerned with linear double stranded DNA, which is coupled to a single support or a tag at the 3′ end of its non-coding strand and methods for producing said linear double stranded DNA. The present invention further relates to the use of said linear double stranded DNA in an RNA in vitro transcription reaction and also to a method for producing RNA in vitro. The present invention also relates to a bioreactor for RNA in vitro transcription.
Claims20
A linear double stranded DNA comprising a coding strand and a non-coding strand, wherein said DNA comprises:
- (i) a coding sequence element in the direction of from 5′ to 3′ of the coding strand, said coding sequence element comprising an open reading frame;
- (ii) an RNA polymerase promotor sequence element upstream of the coding sequence element;
- and (iii) a sequence positioned at the 3′ end of the coding sequence element which encodes a 3′ untranslated region (UTR), said 3′ UTR comprising a poly-A sequence of at least 50 adenosines, wherein said non-coding strand is coupled at its 3′ end to a support or a tag, and wherein said support or tag is the only support or tag coupled to said DNA.
Dependencies are inferred from the claim text.