Preparation and application of high-activity and high-safety cardamine violifolia selenoprotein standard sample

A preparation method to obtain highly safe and active selenoprotein from Cardamine violifolia, the preparation method comprising the following steps:

• step 1, hydrolyzing Cardamine violifolia powder with cellulase, performing cellulase deactivation treatment after the hydrolyzing, and then centrifuging to obtain a supernatant;
• step 2, adding an enzyme mixture comprising alkaline protease, trypsin, papain, protease K and protease XIV into the supernatant to perform enzymolysis and simultaneously performing electrodialysis on the supernatant mixture to remove heavy metal ions, inorganic selenium salt ions and then performing enzyme deactivation to obtain a reaction solution;
• step 3, concentrating and drying the reaction solution obtained in step 2 to obtain the selenoprotein in Cardamine violifolia.