US 12,385,060 B2Granted

Compositions and methods for improving crop yields through trait stacking

AssigneeMonsanto Technology LLC

InventorsCharles R. Dietrich, Natalia Ivleva, Thomas L. Slewinski

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Abstract

The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits and yield relative to a control plant, such as increased ear area, increased ear volume, increased single kernel weight, increased ear fresh weight, increased ear diameter, increased ear length, increased yield, increased kernels per ear, decreased ear tip void, and decreased ear void. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptides and a transgene encoding one or more MADS-box polypeptides, and can also have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Claims26

Dependent claims

A modified corn plant or a plant part thereof comprising 1) a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:

9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, 2) a second recombinant expression cassette comprising a first DNA sequence encoding a CONSTANS-like (COL) polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168, and 3) a third recombinant expression cassette comprising a second DNA sequence encoding a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes.

2 more elements

A method for producing a modified corn plant, the method comprising a method selected from the group consisting of (1)-(17):

(1) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168, wherein the corn cell comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, and a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second and third recombinant expression cassettes;
and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(2) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176, wherein the corn cell comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, and a third recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second, and third recombinant expression cassettes;
and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(3) a. introducing into a corn cell a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 and a third recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second, and third recombinant expression cassettes;
and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassette;
(4) a. introducing into a corn cell 1) a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, 2) a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168, and 3) a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second, and third recombinant expression cassettes;
and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(5) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the first modified corn plant relative to a wildtype control, and wherein the second modified corn plant comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 and a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes;
wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(6) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168, wherein the second modified corn plant comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the second modified corn plant relative to a wildtype control, and wherein the second modified corn plant comprises a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes;
wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(7) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176, wherein the second modified corn plant comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the second modified corn plant relative to a wildtype control, and wherein the second modified corn plant comprises a third recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168;
and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes;
wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(8) a. performing one or more crossings using one or more modified corn plants, wherein the one or more modified corn plants are selected from the group consisting of:
i. a modified corn plant comprising a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the modified corn plant relative to a wildtype control,
ii. a modified corn plant comprising a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168,
iii. a modified corn plant comprising a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176,
iv. a modified corn plant comprising a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the modified corn plant relative to a wildtype control, and comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168,
v. a modified corn plant comprising a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the modified corn plant relative to a wildtype control, and comprises a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176, and
vi. a modified corn plant comprising a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 and a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176, and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes;
wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(9) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises (1) one or more mutations and/or edits in one or more endogenous GA20 oxidase genes and (2) a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 operably linked to a second plant-expressible promoter;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes and the one or more mutations and/or edits, and wherein the level of expression or activity of the one or more endogenous GA20 oxidase genes in the modified corn plant is reduced relative to a control plant not having the one or more mutations and/or edits;
(10) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises (1) one or more mutations and/or edits in one or more endogenous GA20 oxidase genes and (2) a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 operably linked to a second plant-expressible promoter;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes and the one or more mutations and/or edits, and wherein the level of expression or activity of the one or more endogenous GA20 oxidase genes in the modified corn plant is reduced relative to a control plant not having the one or more mutations and/or edits;
(11) a. mutating or editing one or more endogenous GA20 oxidase genes in a corn cell, wherein the corn cell comprises (1) a first recombinant expression cassette comprising a encoding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 operably linked to a first plant-expressible promoter and (2) a second recombinant expression cassette comprising a encoding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 operably linked to a second plant-expressible promoter;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes and the one or more mutations and/or edits, and wherein the level of expression or activity of the one or more endogenous GA20 oxidase genes in the modified corn plant is reduced relative to a control plant not having the one or more mutations and/or edits;
(12) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168, wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes;
(13) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176, wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes;
wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes;
(14) a. introducing into a corn cell 1) a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168, and 2) a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes;
wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes;
(15) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168, and wherein the second modified corn plant comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176;
and
b. producing a progeny corn plant comprising the first and second recombinant expression cassettes;
wherein the progeny corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes;
(16) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 operably linked to a second plant-expressible promoter;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes;
wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes;
and
(17) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 operably linked to a second plant-expressible promoter;
and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes;
wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes.

125 more elements

A recombinant DNA construct comprising:

a. 1) a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:
15, and wherein the transcribable DNA sequence is operably linked to a first plant-expressible promoter, 2) a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 operably linked to a second plant-expressible promoter, and 3) a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 operably linked to a third plant-expressible promoter;
or
b. a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
168 operably linked to a first plant-expressible promoter, and a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
176 operably linked to a second plant-expressible promoter.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/634,381Filed 13 Aug 2020 · us provisional application
Priority: 14 Aug 2019
Published: US 2022/0298527 A122 Sep 2022
Granted: 12 Aug 2025
Term ends: Not estimatedThe source records don’t support an estimate.

Classification13 codes

C12N 15/8297
Hormone-influenced development: gibberellins; GA3
C12Y 114/11012
With 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors: gibberellin-44 dioxygenase
A01H 6/4684
Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize: zea mays [maize]
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants