Yeast strains exhibiting prolonged persistence during a plurality of fermentation cycles
AssigneeDanstar Ferment AG
InventorsEmily Agnes Stonehouse, John McBride, Justin Van Rooyen, Zach Losordo, Petra Deane
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Abstract
The present disclosure provides yeasts, which can be recombinant yeast host cells, exhibiting prolonged persistence when submitted to a plurality of fermentation cycles. The yeasts exhibit at least one of the following phenotypic trait: a fast settling phenotype, a rugose phenotype, an improved invertase activity, triploidy, increased signaling in a RAS/cAMP/PKA pathway or combinations thereof.
Claims23
A recombinant yeast host cell expressing a heterologous sugar transporter protein (STL1) and/or having, when compared to a parental cell, a decreased expression of a native NAD-dependent glycerol-3-phosphate dehydrogenase gene,
- wherein the heterologous sugar transporter protein comprises the amino acid sequence of any one of SEQ ID NO:
- 8 and 31 to 69, is a variant having at least 70% identity to the amino acid sequence of any one of SEQ ID NO:
- 8 and 31 to 69 and glycerol transport activity, or is a fragment having at least 70% identity to the amino acid sequence of any one of SEQ ID NO:
- 8 and 31 to 69 and glycerol transport activity;
- and
- wherein the recombinant yeast host cell has at least one of phenotypic trait providing persistence of the recombinant yeast host cell in a plurality of fermentation cycles, and wherein the at least one phenotypic trait is triploidy.
Dependencies are inferred from the claim text.