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US 12,385,046 B2Granted
Baicalein- and scutellarein-synthesizing microorganism, preparation method and applications thereof
AssigneeCas Center for Excellence In Molecular Plant Sciences
InventorsYong Wang, Jianhua Li, Chenfei Tian
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Abstract
Provided are a baicalein- and scutellarein-synthesizing microorganism, a preparation method for same, and applications thereof. By modifying a heterologous metabolic pathway of a host cell per a genetic engineering method, acquired is an engineered strain providing a high yield of baicalein and scutellarein. Also provided is a process for utilizing the engineered strain to produce baicalein and scutellarein.
Claims7
A method of producing baicalein and scutellarein, comprising:
- (1) introducing genes expressing flavone 6-hydroxylase and cytochrome P450 oxidoreductase into a host cell to obtain a recombinant strain, wherein said flavone 6-hydroxylase is a mutant flavone 6-hydroxylase with the N-terminal amino acids 2 to 25 truncated and fused with a peptide tag, said peptide tag is a 2B1 family soluble protein of cytochrome P450, and said host cell is a Escherichia coli cell;
- (2) culturing the recombinant host cell in a culture system containing chrysin or apigenin to produce baicalein or scutellarein.
A method of producing baicalein and scutellarein, comprising:
- (1) introducing genes expressing flavone 6-hydroxylase and cytochrome P450 oxidoreductase, as well as genes for synthesizing chrysin or apigenin, into a host cell, wherein said flavone 6-hydroxylase is a mutant flavone 6-hydroxylase with the N-terminal amino acids 2 to 25 truncated and fused with a peptide tag, said peptide tag is a 2B1 family soluble protein of cytochrome P450, and said host cell is a Escherichia coli cell;
- (2) culturing the host cell in a culture system containing phenylalanine and/or tyrosine to produce baicalein or scutellarein.
Dependencies are inferred from the claim text.