Promoters and compositions
AssigneeOxford University Innovation Limited
InventorsHagan Bayley, Michael Booth
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Abstract
The present invention relates to activatable promoters, as well as expression systems, vectors, delivery systems, reaction vessels, aqueous objects and compositions comprising said activatable promoters. The invention also provides compositions comprising assemblies and networks of aqueous objects comprising said activatable promoters. The invention additionally provides methods of expressing a transcript and methods of expressing a polypeptide using the activatable promoters of the invention. The invention further provides phospholipid mixtures, as well as aqueous objects and compositions comprising said phospholipid mixtures.
Claims29
An activatable promoter comprising a nucleotide promoter sequence and a transcription inhibition moiety, wherein:
- a) the transcription inhibition moiety comprises a complex of a protein and small molecule;
- b) the small molecule is coupled to a component of the nucleotide promoter sequence via a photocleavable moiety, wherein the component of the nucleotide promoter sequence is one or more nucleotide bases or nucleotide base analogues, and wherein the one or more nucleotide bases or nucleotide base analogues comprises a thymine;
- c) the photocleavable moiety comprises a nitroaryl group and a diamine linker, and the transcription inhibition moiety and the photocleavable moiety are coupled to the thymine via the diamine linker, and the small molecule is coupled to the diamine linker via the nitroaryl group;
- d) the diamine linker and thymine form a C6-amino-dT or a C6-amino-T;
- and
- e) the nucleotide promotor sequence is configured to be activated when the transcription inhibition moiety is uncoupled from the activatable promoter by cleavage of the photocleavable moiety.
Dependencies are inferred from the claim text.